Laboratory technician for the research group “RNA-based Regulation of Gene Expression”

About the Position

Laboratory Technician for the Research Group “RNA-based Regulation of Gene Expression”

We are seeking a dedicated Laboratory Technician to join the research group of Peter J Lukavsky, PhD, which is part of the Structural Biology program at CEITEC, Masaryk University (MU). Our group focuses on studying disease-related biological processes by investigating protein structures and their interactions with other proteins, RNA, or small molecule inhibitors using biomolecular NMR and related biophysical methods.

Responsibilities

• Expression and purification of proteins
• Transcription and purification of RNAs
• Preparation of various media and buffers
• PCR and cloning of RNA and protein constructs
• Preparation of isotope-labelled nucleotides
• Binding studies using ITC, fluorescence anisotropy, EMSA, and other biophysical techniques including data analysis
• Performance of NMR experiments to study small molecule interactions with proteins and RNA
• Implementing new techniques and projects
• Help incoming co-workers into a smooth start in the lab
• Ordering of chemicals and lab supplies

Requirements

• At least a Master's degree in molecular biology, biochemistry, biophysics, chemistry, or other related fields of life sciences
• Good knowledge of English (oral and written)
• Excellent interpersonal and communication skills
• High motivation, flexibility, and self-driven interest to learn new things
• Reliability, team player, meticulousness
• Knowledge of "laboratory chemistry" - preparation of solutions with defined concentrations, adjusting pH, preparation of media for bacterial cultures, etc.
• Computer literacy, MS Word, Excel, Adobe Illustrator
• Excellent experience with molecular biology, protein and RNA purification, biochemistry, and NMR spectroscopy is a big plus

We Offer

• Interesting work in a top scientific team
• Stable base and excellent instrumental equipment of CEITEC-MU
• MU employment benefits
• Salary based on qualification and experience; part-time appointment possible

Work Conditions

• Medical certificate required
• Personal protective equipment not provided

About the Company

CEITEC MU is a leading research institution in the Czech Republic, focusing on cutting-edge research in structural biology and related fields.

Application Process

Please send your CV and a short motivational letter including:

• Description of previous experiences
• Technical skills
• At least two contact references

Applications should be sent via the e-application available on CEITEC MU Careers.

Deadline for applications: 31 August 2022.

For any difficulties filling out the e-application, please contact Veronika Slezáková.

Location: Brno, Czech Republic

Salary: 28,000 CZK per month

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